THESIS OPPORTUNITIES IN ELECTROPHYSIOLOGY LABORATORY

 

(Electrophysiology Laboratory was founded by Zsolt Kovács

and Gábor Juhász in 2001, Department of Zoology, University of West Hungary,

Savaria Campus, Szombathely)

 

1. Links between neuroimmunological reactions and pathomechanism of epilepsy (WAG/Rij rats);
2. Effect of exogenous ketone supplementation on epileptic seizures;
3. Effects of nucleosides on absence epileptic activity (WAG/Rij rats).

 

FROM WAG/RIJ RAT TO SWD DETECTION

AND PUBLICATION OF RESULTS

 

SCIENTIFIC WORK IN ELECTROPHYSIOLOGY LABORATORY

(Department of Zoology, University of West Hungary,

Savaria Campus, Szombathely)

 

All animal treatments and surgery procedures were carried out in correspondence with the local ethical rules which are in conformity with the guidelines of the European Communities Council Directive 24 November 1986 (86/609/EEC) to use and treat animals in experimental laboratories.

 

I.

METHODS: ELECTRODE IMPLANTATION
INTO THE RAT SKULL AND EEG RECORDING

Preparing of surgical tools to surgery

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DEMONSTRATION OF ELECTRODES

AND THEIR PLACES

 

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ELECTRODE IMPLANTATION AND

EXPERIMENTAL PARADIGM

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Kovács, Z., et al., 2015, Neuroscience, 300, 593-608.,  http://www.sciencedirect.com/science/article/pii/S0306452215004960

 

EEG RECORDING

After injection of drugs, recording was carried out in an electrically shielded and soundproof box (Faraday-box). EEG was recorded by a differential preamplifier (SUPERTECH Bioamp 4, Hungary or NIHON-COHDEN EEG, Japan) attached to a CED 1401 mkII a data capture and analysis device using SPIKE 2 software of the company (Cambridge, UK). The bandwidth of the EEG recording was 0.53 Hz to 150 Hz and it was sampled at 1 kHz sampling rate.

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The selected spike-wave discharges (SWDs) were cut off from

 the raw data files (EEG) and checked by FFT analysis.

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II.

EVALUATION OF RESULTS

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Kovács, Z., et al., 2015, Neuroscience, 300, 593-608.,  http://www.sciencedirect.com/science/article/pii/S0306452215004960

 

III.

PUBLICATION OF RESULTS

Effects of nucleosides on absence epileptic activity based on the literature.

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Kovács, Z., et al., 2015. Mini Rev. Med. Chem., 14(13), 1033-42. http://www.eurekaselect.com/125918/article

 

 

Effects of nucleosides on absence epileptic activity based on our results.

../Képek/10

 

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You can find it here:

1. Kovács, Z., Kékesi, K.A., Juhász, G., Dobolyi, A. (2015). Modulatory effects of inosine, guanosine and uridine on lipopolysaccharide-evoked increase in spike-wave discharge activity in Wistar Albino Glaxo/Rijswijk rats. Brain Res. Bull. 118, 46-57. (IF 2014: 2,718) http://www.sciencedirect.com/science/article/pii/S0361923015300319

2. Kovács, Z., Kékesi, K.A., Dobolyi, Á., Lakatos, R., Juhász, G. (2015). Absence epileptic activity changing effects of non-adenosine nucleoside inosine, guanosine and uridine in Wistar Albino Glaxo Rijswijk rats. Neuroscience, 300, 593-608. (IF 2014: 3,357) http://www.sciencedirect.com/science/article/pii/S0306452215004960

3. Kovács, Z., Dobolyi, Á., Juhász, G., Kékesi, K.A. (2014). Lipopolysaccharide induced increase in seizure activity in two animal models of absence epilepsy WAG / Rij and GAERS rats and Long Evans rats. Brain Res. Bull. 104: 7-18. (IF: 2,718) http://www.sciencedirect.com/science/article/pii/S0361923014000392

4. Kovács, Z., Slézia, A., Bali, Z. K., Kovács, P., Dobolyi, Á., Szikra, T., Hernádi, I., Juhász. G. (2013). Uridine modulates neuronal activity and inhibits spike-wave discharges of absence epileptic Long Evans and Wistar Albino Glaxo/Rijswijk rats. Brain Res. Bull. 97, 16-23. (IF 2,974) http://www.sciencedirect.com/science/article/pii/S0361923013000919

5. Kovács, Z., Czurkó, A., Kékesi, K. A., Juhász, G. (2011). Intracerebroventricularly administered lipopolysaccharide enhances spike-wave discharges in freely moving WAG/Rij rats. Brain Res. Bull. 85, 410-416. (IF 2,818) http://www.sciencedirect.com/science/article/pii/S0361923011001468

6. Kovács, Z., Kékesi, K.A., Szilágyi, N., Ábrahám, I., Székács, D., Király, N., Papp, E., Császár, I., Szego, E., Barabás, K., Péterfy, H., Erdei, A., Bártfai, T., Juhász, G. (2006). Facilitation of spike-wave discharge activity by lipopolysaccharides in Wistar Albino Glaxo/Rijswijk rats. Neuroscience 140:731-742. (IF 3.427) http://www.ncbi.nlm.nih.gov/pubmed/16616432